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PINK1-SPARK
(Plasmid #248086)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248086 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3
  • Total vector size (bp) 7126
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PINK1-SPARK
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Original SPARK construct was developed by Xiaokun Shu's lab (Addgene #106920)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.08.681260 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PINK1-SPARK was a gift from Danielle Schmitt (Addgene plasmid # 248086 ; http://n2t.net/addgene:248086 ; RRID:Addgene_248086)

  • For your References section:

    Visualizing PINK1 Activity Dynamics in Single Cells with a Phase Separation-Based Kinase Activity Reporter. Vineall KG, Andrikopoulos A, Sun MJ, Yan A, Hartanto ER, Schmitt DL. bioRxiv [Preprint]. 2025 Oct 16:2025.10.08.681260. doi: 10.1101/2025.10.08.681260. 10.1101/2025.10.08.681260 PubMed 41279391
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