Skip to main content
Addgene
Open Menu

pSHARK2
(Plasmid #248168)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248168 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pBBR1-SpecR
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB10Beta
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LambdaCI-PirWT
  • Species
    E. coli
  • Mutation
    None

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.06.680659 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSHARK2 was a gift from Thomas Gorochowski (Addgene plasmid # 248168 ; http://n2t.net/addgene:248168 ; RRID:Addgene_248168)

  • For your References section:

    SHARK: A Specialized Host for Assembling R6K Plasmids. Hina-Nilesh Joshi S, Jenkins C, Ulaeto D, Gorochowski TE. ACS Synth Biol. 2026 Jan 13. doi: 10.1021/acssynbio.5c00749. 10.1021/acssynbio.5c00749 PubMed 41529903
Related items: