pRK415-mNeonGreen (constitutive expression)
(Plasmid
#248204)
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PurposeConstitutive expression of mNeonGreen with a very low copy F-like plasmid under the control of a lac promoter. Plasmid contained within E. coli TOP10 (for cloning)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRK415
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Backbone manufacturerNova Lifetech Inc.
- Backbone size w/o insert (bp) 10682
- Total vector size (bp) 11392
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Modifications to backbonemNeonGreen
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Vector typeBacterial Expression, Synthetic Biology ; very low copy
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Selectable markersTetracycline
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Growth instructionsLB Miller
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemNeonGreen
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SpeciesSynthetic; Branchiostoma lanceolatum
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Insert Size (bp)711
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GenBank IDKC295282.1
- Promoter lac
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK415-mNeonGreen (constitutive expression) was a gift from Cecile Fradin (Addgene plasmid # 248204 ; http://n2t.net/addgene:248204 ; RRID:Addgene_248204)
Map uploaded by the depositor.
