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pANC61(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron-IRES-EGFP)
(Plasmid #248354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248354 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron
  • Alt name
    DD-URCAS sensor
  • Species
    H. sapiens (human), Synthetic
  • Promoter EF-1a
  • Tags / Fusion Proteins
    • FLAG (internal in Aqp1)
    • 3x TEV site, FKBP12 degron (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DD-URCAS: destabilizing domain universal reporter circuit-based activatable sensor. Please visit https://doi.org/10.1101/2025.09.07.674790 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pANC61(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron-IRES-EGFP) was a gift from Arnab Mukherjee (Addgene plasmid # 248354 ; http://n2t.net/addgene:248354 ; RRID:Addgene_248354)

  • For your References section:

    A programmable genetic platform for engineering noninvasive biosensors. Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K, Tiwari R, Mukherjee A. bioRxiv 2025.09.07.674790 10.1101/2025.09.07.674790
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