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pEG-MeCP2_R294X (pc4753)
(Plasmid #248580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248580 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5570
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MeCP2
  • Species
    H. sapiens (human)
  • Mutation
    aa1-aa293
  • Entrez Gene
    MECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.14.643219 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG-MeCP2_R294X (pc4753) was a gift from Cristina Cardoso (Addgene plasmid # 248580 ; http://n2t.net/addgene:248580 ; RRID:Addgene_248580)

  • For your References section:

    MeCP2-driven chromatin organization controls nuclear stiffness. Romero H, Amiri A, Pabba MK, Zhang H, Berg V, Arroyo M, Prorok P, Zhadan A, Mahmoud M, Trautwein N, Laube B, Dietz C, Stark RW, Cardoso MC. Commun Biol. 2025 Dec 8. doi: 10.1038/s42003-025-09328-6. 10.1038/s42003-025-09328-6 PubMed 41360929
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