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pCMV-SNAP-Rab6a_T27N
(Plasmid #248794)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248794 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV-intron myc
  • Backbone size w/o insert (bp) 3800
  • Total vector size (bp) 4898
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab6a
  • Species
    H. sapiens (human)
  • Mutation
    Change T 27 to N
  • Entrez Gene
    RAB6A (a.k.a. RAB6)
  • Tag / Fusion Protein
    • SNAPf (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    To generate the SNAP-Rab6a-T27N plasmid, we replaced the N-terminal myc tag sequences in the PCMV-intron myc Rab6 T27N (Addgene #46782) plasmids with the SNAPf sequence from the SNAP-Sec61B plasmid (Addgene #5520S) using HiFi DNA Assembly cloning.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SNAP-Rab6a_T27N was a gift from Michael Robichaux (Addgene plasmid # 248794 ; http://n2t.net/addgene:248794 ; RRID:Addgene_248794)

  • For your References section:

    Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor Golgi. Hekmatara M, Thompson SL, Haggerty KN, Hagen S, Brothers BA, Daniels B, Luxardi G, Moshiri A, Deng WT, Robichaux MA. bioRxiv [Preprint]. 2025 Sep 25:2025.09.24.678292. doi: 10.1101/2025.09.24.678292. 10.1101/2025.09.24.678292 PubMed 41040232
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