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TDP-43_CTD_12S→E_S305C/A326P
(Plasmid #248868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248868 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTHMT
  • Backbone size w/o insert (bp) 6458
  • Total vector size (bp) 6905
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TDP-43_CTD_12S→E_S305C/A326P
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    447
  • Mutation
    changed S292E, S369E, S377E, S379E, S387E, S389E, S393E, S395E, S403E, S404E, S309E, S410E and S305C, A326P
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Tag / Fusion Protein
    • 6xHis tags-MBP (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.05.602258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TDP-43_CTD_12S→E_S305C/A326P was a gift from Nicolas Fawzi (Addgene plasmid # 248868 ; http://n2t.net/addgene:248868 ; RRID:Addgene_248868)

  • For your References section:

    Structural details of helix-mediated multimerization of the conserved region of TDP-43 C-terminal domain. Rizuan A, Shenoy J, Mohanty P, Dos Passos PM, Mercado Ortiz JF, Bai L, Viswanathan R, Zaborowksy J, Wang SH, Johnson V, Mamede LD, Titus AR, Ayala YM, Ghirlando R, Mittal J, Fawzi NL. Nat Commun. 2025 Nov 26;16(1):10528. doi: 10.1038/s41467-025-65546-w. 10.1038/s41467-025-65546-w PubMed 41298366
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