Skip to main content
Addgene
Open Menu

pcDNA4-MS2-CP-GFP-SBP
(Plasmid #249048)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249048 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pcDNA4
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MS2 binding coat protein
  • Species
    MS2 bacteriophage
  • GenBank ID
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • streptavidin binding peptide (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-MS2-CP-GFP-SBP was a gift from Jeffrey Gerst (Addgene plasmid # 249048 ; http://n2t.net/addgene:249048 ; RRID:Addgene_249048)

  • For your References section:

    A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes. Slobodin B, Gerst JE. RNA. 2010 Nov;16(11):2277-90. doi: 10.1261/rna.2091710. Epub 2010 Sep 28. 10.1261/rna.2091710 PubMed 20876833
Related items: