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pTYB1-MeCP2-R255X (pc4735)
(Plasmid #249082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249082 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTYB1
  • Backbone manufacturer
    NEB
  • Total vector size (bp) 8209
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MeCP2
  • Species
    H. sapiens (human)
  • Mutation
    MeCP2-R255X (aa1-aa254)
  • Entrez Gene
    MECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTYB1-MeCP2-R255X (pc4735) was a gift from Cristina Cardoso (Addgene plasmid # 249082 ; http://n2t.net/addgene:249082 ; RRID:Addgene_249082)

  • For your References section:

    MeCP2-induced heterochromatin organization is driven by oligomerization-based liquid-liquid phase separation and restricted by DNA methylation. Zhang H, Romero H, Schmidt A, Gagova K, Qin W, Bertulat B, Lehmkuhl A, Milden M, Eck M, Meckel T, Leonhardt H, Cardoso MC. Nucleus. 2022 Dec;13(1):1-34. doi: 10.1080/19491034.2021.2024691. 10.1080/19491034.2021.2024691 PubMed 35156529
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