pEGFP-N1/Integrin-b3-Halo
(Plasmid #249172)

• Purpose: Mammalian expression vector of human ITGB3 fused with Halo-tag
• Depositing Lab: Pakorn Kanchanawong
• Publication: Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6277
• Total vector size (bp): 8638
• Modifications to backbone: EGFP was replaced with Halo-tag
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrin beta-3
• Species: H. sapiens (human)
• Insert Size (bp): 2361
• Entrez Gene: ITGB3 (a.k.a. BDPLT16, BDPLT2, BDPLT24, CD61, FMAIT1, GP3A, GPIIIa, GT, GT2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Halo (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • integrin-beta3-halo.gb
• A portion of this plasmid was derived from a plasmid made by: Integrin-beta3-PAGFP from Dr. Michael Sheetz (Changede et al., 2019)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.04.17.649301 for bioRxiv preprint.