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mEmerald-myo1e
(Plasmid #249264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249264 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    mEmerald-C1
  • Backbone manufacturer
    Michael Davidson (Addgene #53975)
  • Backbone size w/o insert (bp) 4750
  • Total vector size (bp) 7983
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myo1e
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYO1E (a.k.a. FSGS6, HuncM-IC, MYO1C)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-forward
  • 3′ sequencing primer M13
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-myo1e was a gift from Mira Krendel (Addgene plasmid # 249264 ; http://n2t.net/addgene:249264 ; RRID:Addgene_249264)

  • For your References section:

    Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis. Barger SR, Reilly NS, Shutova MS, Li Q, Maiuri P, Heddleston JM, Mooseker MS, Flavell RA, Svitkina T, Oakes PW, Krendel M, Gauthier NC. Nat Commun. 2019 Mar 19;10(1):1249. doi: 10.1038/s41467-019-09104-1. 10.1038/s41467-019-09104-1 PubMed 30890704
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