pBabepuro3-p18Flag
(Plasmid #24933)

• Depositing Lab: Lynda Chin
• Publication: Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Backbone

• Vector backbone: pBabe puro3
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: P18
• Species: H. sapiens (human)
• Entrez Gene: CDKN2C (a.k.a. INK4C, p18, p18-INK4C)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: pBABE-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr Christoph Geisen

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry