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pBabepuro3-p18Flag
(Plasmid #24933)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24933 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe puro3
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P18
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDKN2C (a.k.a. INK4C, p18, p18-INK4C)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Christoph Geisen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabepuro3-p18Flag was a gift from Lynda Chin (Addgene plasmid # 24933 ; http://n2t.net/addgene:24933 ; RRID:Addgene_24933)
  • For your References section:

    Feedback circuit among INK4 tumor suppressors constrains human glioblastoma development. Wiedemeyer R, Brennan C, Heffernan TP, Xiao Y, Mahoney J, Protopopov A, Zheng H, Bignell G, Furnari F, Cavenee WK, Hahn WC, Ichimura K, Collins VP, Chu GC, Stratton MR, Ligon KL, Futreal PA, Chin L. Cancer Cell. 2008 Apr . 13(4):355-64. 10.1016/j.ccr.2008.02.010 PubMed 18394558