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Suntag_dPRDM9_FWA_g4
(Plasmid #249562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249562 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEG302
  • Backbone size w/o insert (bp) 25324
  • Total vector size (bp) 26248
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRDM9
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    924
  • Mutation
    G282A (Numbering from the the full length sequence)
  • Entrez Gene
    Prdm9 (a.k.a. Dsbc1, G1-419-29, Meisetz, PRDM9-B, Rcr1, repro7)
  • Promoter UBQ10
  • Tags / Fusion Proteins
    • sfGFP (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Suntag_dPRDM9_FWA_g4 was a gift from Jake Harris (Addgene plasmid # 249562 ; http://n2t.net/addgene:249562 ; RRID:Addgene_249562)

  • For your References section:

    CRISPR targeting of H3K4me3 activates gene expression and unlocks centromere-proximal crossover recombination in Arabidopsis. Binenbaum J, Adamkova V, Fryer H, Xu L, Gorringe N, Wlodzimierz P, Burns R, Papikian A, Jacobsen SE, Henderson IR, Harris CJ. Nat Commun. 2025 Oct 31;16(1):9587. doi: 10.1038/s41467-025-65167-3. 10.1038/s41467-025-65167-3 PubMed 41173880
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