pET28g
(Plasmid
#249630)
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Purpose(Empty Backbone) Clone genes for protein expression in Escherichia coli using Golden Gate cloning and the pET28a backbone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28g was a gift from Isabel Abreu (Addgene plasmid # 249630 ; http://n2t.net/addgene:249630 ; RRID:Addgene_249630) -
For your References section:
pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2. Luis IM, Parada M, Vicente JB, Abreu IA. PLoS One. 2025 Jul 7;20(7):e0327341. doi: 10.1371/journal.pone.0327341. eCollection 2025. 10.1371/journal.pone.0327341 PubMed 40622926