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p028g13-Gb1
(Plasmid #249632)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249632 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAGM9121
  • Backbone manufacturer
    MoClo Toolkit, Addgene Kit #1000000044
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-terminal tag with 8x His-residues in tandem with Gb1 polypeptide
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p028g13-Gb1 was a gift from Isabel Abreu (Addgene plasmid # 249632 ; http://n2t.net/addgene:249632 ; RRID:Addgene_249632)

  • For your References section:

    pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2. Luis IM, Parada M, Vicente JB, Abreu IA. PLoS One. 2025 Jul 7;20(7):e0327341. doi: 10.1371/journal.pone.0327341. eCollection 2025. 10.1371/journal.pone.0327341 PubMed 40622926
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