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pTol2-HuC_SiR-tag-Pro30-mEGFP-NLS3x
(Plasmid #249672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249672 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTol2
  • Backbone size w/o insert (bp) 12500
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SiR-tag-Pro30-mEGFP-NLS3x
  • Species
    Chlorobaculum tepidum TLS

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2-HuC_SiR-tag-Pro30-mEGFP-NLS3x was a gift from Kai Johnsson & Richard Wombacher (Addgene plasmid # 249672 ; http://n2t.net/addgene:249672 ; RRID:Addgene_249672)

  • For your References section:

    Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins. Kompa J, Dornfeld LJ, Porzberg N, Jang S, Zedlitz S, Lilje SH, Catapano C, Jocher D, Merk L, Hoege C, Mao R, Wilhelm J, Dietz MS, Tarnawski M, Hiblot J, Hyman AA, Heilemann M, Johnsson K. J Am Chem Soc. 2025 Dec 30. doi: 10.1021/jacs.5c18083. 10.1021/jacs.5c18083 PubMed 41468521
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