M_3HN5SR
(Plasmid
#249741)
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PurposeMinimal synthetic construct for assessing speckle localization containing a 3' splice site, an HNRNP motif-rich region, 5' splice site, and an SR motif-rich region
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249741 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUHD10-3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM_3HN5SR
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SpeciesSynthetic
- Promoter TRE+CMV
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M_3HN5SR was a gift from Oded Regev (Addgene plasmid # 249741 ; http://n2t.net/addgene:249741 ; RRID:Addgene_249741) -
For your References section:
RNA localization to nuclear speckles follows splicing logic. Wen L, Arias MA, Fan X, Paul S, Liao SE, Sobczyk M, Regev O, Fei J. bioRxiv [Preprint]. 2025 May 28:2025.05.28.656493. doi: 10.1101/2025.05.28.656493. 10.1101/2025.05.28.656493 PubMed 40501903