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U1_snRNA_Weak_SS
(Plasmid #249810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249810 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pU1_000 (Addgene #186773)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mutated human U1 snRNA
  • Species
    Synthetic
  • Promoter U1

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U1_snRNA_Weak_SS was a gift from Oded Regev (Addgene plasmid # 249810 ; http://n2t.net/addgene:249810 ; RRID:Addgene_249810)

  • For your References section:

    RNA localization to nuclear speckles follows splicing logic. Wen L, Arias MA, Fan X, Paul S, Liao SE, Sobczyk M, Regev O, Fei J. bioRxiv [Preprint]. 2025 May 28:2025.05.28.656493. doi: 10.1101/2025.05.28.656493. 10.1101/2025.05.28.656493 PubMed 40501903
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