|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24993||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
DNA encoding the catalytic domain of xynB from C. stercorarium NCIMB 11754 was synthesized and codon-optimized by DNA 2.0. XynB was cloned using an EcoRI site on the 5' end and a BamHI site on the 3' end.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGB3 was a gift from Jay Keasling (Addgene plasmid # 24993 ; http://n2t.net/addgene:24993 ; RRID:Addgene_24993)
For your References section:Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD.. Nature. 2010 Jan 28;463(7280):559-62. 10.1038/nature08721 PubMed 20111002