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palm-Bassoon
(Plasmid #250162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250162 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4204
  • Total vector size (bp) 15817
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    palmitoylated Bassoon construct (N-terminal 94 aa replaced by palmitoylation consensus)
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    11613
  • Entrez Gene
    Bsn (a.k.a. Znf231)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer CAACGGGACTTTCCAAAATG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

1) Identification of Bassoon: https://doi.org/10.1083/jcb.142.2.499
2) First description of a full-length recombinant Bassoon construct: https://doi.org/10.1016/S1044-7431(03)00015-0

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    palm-Bassoon was a gift from Thomas Dresbach (Addgene plasmid # 250162 ; http://n2t.net/addgene:250162 ; RRID:Addgene_250162)

  • For your References section:

    Establishing synthetic ribbon-type active zones in a heterologous expression system. Kapoor R, Do TT, Schwenzer N, Petrovic A, Dresbach T, Lehnart SE, Fernandez-Busnadiego R, Moser T. Elife. 2026 Jan 13;13:RP98254. doi: 10.7554/eLife.98254. 10.7554/eLife.98254 PubMed 41528127
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