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pJC2528 - CLYBL-Neo-CAGGS-LSL-dCas9-TagBFP-KRAB-CLYBL (LSL-CRISPRi)
(Plasmid #250253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250253 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pC13N-dCas9-BFP-KRAB
  • Backbone manufacturer
    Martin Kampmann (Addgene Plasmid #127968)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-mTagBFP-KRAB
  • Species
    Synthetic
  • Promoter CAGGS
  • Tag / Fusion Protein
    • HA-2xSV40 NLS-mTagBFP-KRAB (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC2528 - CLYBL-Neo-CAGGS-LSL-dCas9-TagBFP-KRAB-CLYBL (LSL-CRISPRi) was a gift from Jacob Corn (Addgene plasmid # 250253 ; http://n2t.net/addgene:250253 ; RRID:Addgene_250253)

  • For your References section:

    C-terminal amides mark proteins for degradation via SCF-FBXO31. Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek M, Schulman BA, Bode JW, Corn JE. Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w. 10.1038/s41586-024-08475-w PubMed 39880951
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