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pBabe-puro-miR-10b sponge
(Plasmid #25043)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25043 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Bob Weinberg, Addgene plasmid 1764
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-10b sponge
  • Insert Size (bp)
    186

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

8 tandem binding sites to human miR-10b. Repeated sequence is CACAAATTCGGAGAACAGGGTA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro-miR-10b sponge was a gift from Bob Weinberg (Addgene plasmid # 25043 ; http://n2t.net/addgene:25043 ; RRID:Addgene_25043)
  • For your References section:

    Therapeutic silencing of miR-10b inhibits metastasis in a mouse mammary tumor model. Ma L, Reinhardt F, Pan E, Soutschek J, Bhat B, Marcusson EG, Teruya-Feldstein J, Bell GW, Weinberg RA. Nat Biotechnol. 2010 Apr . 28(4):341-7. 10.1038/nbt.1618 PubMed 20351690