|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25043||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBob Weinberg, Addgene plasmid 1764
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemiR-10b sponge
Insert Size (bp)186
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
8 tandem binding sites to human miR-10b. Repeated sequence is CACAAATTCGGAGAACAGGGTA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe-puro-miR-10b sponge was a gift from Bob Weinberg (Addgene plasmid # 25043 ; http://n2t.net/addgene:25043 ; RRID:Addgene_25043)
For your References section:Therapeutic silencing of miR-10b inhibits metastasis in a mouse mammary tumor model. Ma L, Reinhardt F, Pan E, Soutschek J, Bhat B, Marcusson EG, Teruya-Feldstein J, Bell GW, Weinberg RA. Nat Biotechnol. 2010 Apr . 28(4):341-7. 10.1038/nbt.1618 PubMed 20351690