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pRS479
(Plasmid #250493)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250493 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRS159
  • Backbone size (bp) 14937
  • Modifications to backbone
    The ACT1 promoter controlling dCAS9 expression was swapped out for a C. albicans optimized tetracycline regulated (tetO) promoter. Additionally, the tetracycline transactivator (TAR) was inserted into the plasmid.
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For transformation into bacteria cells, the transformants must be grown on media containing both Ampicillin (100 μg/mL) and Nourseothricin (50 μg/mL).
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.19.695556 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS479 was a gift from Rebecca Shapiro (Addgene plasmid # 250493 ; http://n2t.net/addgene:250493 ; RRID:Addgene_250493)

  • For your References section:

    Pooled CRISPRi screening reveals fungal-specific drug target candidates. Wensing LF, Després PC, Francis D, Fogal M, Hendriks A, Gervais NC, Fikry C, Adamu Bukari A-R, Gerstein AC, Cuomo CA, Shapiro RS. Nat Microbiol (2026). doi: https://doi.org/10.1038/s41564-026-02356-w 10.1038/s41564-026-02356-w
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