pRSETb 6xHis-Cystector (Cysteine biosensor)
(Plasmid
#250652)
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PurposeE. coli expression plasmid for 6xHisTag-Cystector (Cysteine biosensor)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRSET-B
- Backbone size w/o insert (bp) 2869
- Total vector size (bp) 4420
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCystector (Cysteine biosensor)
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SpeciesNeisseria gonorrhoae + Aequorea victoria
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Insert Size (bp)1551
- Promoter T7 promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.01.27.698081 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETb 6xHis-Cystector (Cysteine biosensor) was a gift from Peter Dedecker (Addgene plasmid # 250652 ; http://n2t.net/addgene:250652 ; RRID:Addgene_250652) -
For your References section:
A genetically-encoded cysteine biosensor to monitor cysteine dynamics across life domains. Pedre B, Deschamps A, Felten C, Leverrier P, Collet J-F, André B, Dedecker P. bioRxiv 2026.01.27.698081 10.64898/2026.01.27.698081