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p416TEF1 Su9-Cystector (Cysteine biosensor)
(Plasmid #250655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250655 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p416TEF1
  • Backbone size w/o insert (bp) 5486
  • Total vector size (bp) 7247
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Su9-Cystector (Cysteine biosensor)
  • Species
    Neurospora crassa + Neisseria gonorrhoae + Aequorea victoria
  • Insert Size (bp)
    1761
  • Promoter TEF1
  • Tag / Fusion Protein
    • Mitochondrial targetting sequence based on Neurospora crassa ATPase subunit 9 (Su9) (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.27.698081 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p416TEF1 Su9-Cystector (Cysteine biosensor) was a gift from Peter Dedecker (Addgene plasmid # 250655 ; http://n2t.net/addgene:250655 ; RRID:Addgene_250655)

  • For your References section:

    A genetically-encoded cysteine biosensor to monitor cysteine dynamics across life domains. Pedre B, Deschamps A, Felten C, Leverrier P, Collet J-F, André B, Dedecker P. bioRxiv 2026.01.27.698081 10.64898/2026.01.27.698081
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