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puDNA5_SSF_DOR_MRS_2Ala_APEX2
(Plasmid #250763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250763 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    puDNA5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DOR-MRS(2Ala)
  • Species
    M. musculus (mouse)
  • Mutation
    delta opioid receptor with last 17 amino acids of MOR appended (both leucines in LENL sequence mutated to alanines)
  • Promoter UBC
  • Tags / Fusion Proteins
    • Signal Sequence FLAG (N terminal on insert)
    • APEX2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    puDNA5_SSF_DOR_MRS_2Ala_APEX2 was a gift from Braden Lobingier (Addgene plasmid # 250763 ; http://n2t.net/addgene:250763 ; RRID:Addgene_250763)

  • For your References section:

    Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complex. Dagunts A, Adoff H, Novy B, Ameur LB, De Maria M, Saunders A, Lobingier BT. Sci Adv. 2026 Mar 20;12(12):eadx8715. doi: 10.1126/sciadv.adx8715. Epub 2026 Mar 20. 10.1126/sciadv.adx8715 PubMed 41861010
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