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pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP
(Plasmid #250765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250765 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VPS35 shRNA
  • gRNA/shRNA sequence
    Unspecified
  • Species
    M. musculus (mouse)
  • Promoter H1 for shRNA, UBC for MOR
  • Tag / Fusion Protein
    • SSF-SNAP(for MOR) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid also encodes SSF-SNAP-MOR(WT) under the UBC promoter, and a P2A GFP under the same promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP was a gift from Braden Lobingier (Addgene plasmid # 250765 ; http://n2t.net/addgene:250765 ; RRID:Addgene_250765)

  • For your References section:

    Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complex. Dagunts A, Adoff H, Novy B, Ameur LB, De Maria M, Saunders A, Lobingier BT. Sci Adv. 2026 Mar 20;12(12):eadx8715. doi: 10.1126/sciadv.adx8715. Epub 2026 Mar 20. 10.1126/sciadv.adx8715 PubMed 41861010
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