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pLX304-Lantern-HaloTag-NES
(Plasmid #250919)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250919 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLX304
  • Backbone size w/o insert (bp) 7656
  • Total vector size (bp) 8979
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lantern
  • Species
    Synthetic
  • Insert Size (bp)
    1323
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (N terminal on insert)
    • HaloTag-NES (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer GGAGGAGAAAATGAAAGCCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-6890342/v1 for ResearchSquare preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304-Lantern-HaloTag-NES was a gift from Peng Zou (Addgene plasmid # 250919 ; http://n2t.net/addgene:250919 ; RRID:Addgene_250919)

  • For your References section:

    Directed evolution of a genetically encoded photocatalyst for temporally resolved proximity labeling of subcellular RNAs and proteins. Fang Y, Ren Z, Zheng F, Wang R, Zhao S, Wang W, Li C, Liu-Yang L, Lin C, Zou P. Research Square 2025 [Preprint]. https://doi.org/10.21203/rs.3.rs-6890342/v1 10.21203/rs.3.rs-6890342/v1
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