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Yeo Lab RNA-Binding Protein Pooled CRISPR Knockout Libraries
(Pooled Library #141438, #250988)

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  • Purpose

    These pooled CRISPR knockout libraries target RNA-binding proteins (RBPs). Pooled library #141438 targets 1,078 RBPs using 10 guide RNAs per gene. Pooled library #250988 targets 206 RBPs with 5 single-guide RNAs (sgRNAs) per gene in the lentiCRISPR v2 backbone, enabling doxycycline-inducible expression of Cas9 after establishment of library-expressing cells in vivo.

    Library #250988 was prioritized from previous CRISPR screens that identified RBPs essential for survival in KRAS-, BRCA1-, and MYC-driven cancers, making it relevant for probing cancer-specific vulnerabilities. Its reduced size, combined with the doxycycline-inducible design of the lentiCRISPR v2 backbone, makes it particularly well-suited for in vivo screening such as within a tumor xenograft context.

  • Vector Backbone

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 141438 RBP CRISPR Knockout Library 1 $594 Add to Cart
Pooled Library 250988 RBP CRISPR Knockout In Vivo Library 1 $418 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Lentiviral Generation
    3rd
Pooled Library Genes Targeted Targeting gRNAs Control gRNAs
141438 1,078 12,472 1,058 non-targeting, 628 essential genes, 12 fluorescent controls
250988 206 1,089 225 non-targeting, 140 essential genes

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RNA-Binding Protein Pooled CRISPR Knockout Library was a gift from Eugene Yeo (Addgene #141438 ; http://n2t.net/addgene:141438 ; RRID:Addgene_141438)
    RNA-Binding Protein Pooled CRISPR Knockout In Vivo Library was a gift from Eugene Yeo (Addgene #250988 ; http://n2t.net/addgene:250988 ; RRID:Addgene_250988)

  • For your References section:

    For #141438:

    Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Wheeler EC, Vu AQ, Einstein JM, DiSalvo M, Ahmed N, Van Nostrand EL, Shishkin AA, Jin W, Allbritton NL, Yeo GW. Nat Methods. 2020 May 11. pii: 10.1038/s41592-020-0826-8. doi: 10.1038/s41592-020-0826-8. PubMed 32393832

    For #250988:

    Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer Progression. Tankka AT, Zhang Y, Einstein JM, Zhou CJ, Pham VN, Naritomi JT, Nguyen GG, Mendez-Molina AN, Hu Z, Mizrahi O, Perelis M, Sarsam J, Tan FE, Kaufman DS, Yang J, Antal CE, Yeo GW. Cancer Res. 2025 Dec 18:10.1158/0008-5472.CAN-25-0453. doi: 10.1158/0008-5472.CAN-25-0453. PubMed 41411621
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