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pGS1T-PylRSC6a-PylTm15CUA
(Plasmid #251554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251554 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGS1T
  • Backbone manufacturer
    in-house design and optimization
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PylRS-C6a
  • Alt name
    PylS-C6a
  • Alt name
    C6aRS
  • Species
    Synthetic
  • Promoter Glutamine synthetase promoter (GlnS)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAAGATCATACGCCGTTATACG
  • 3′ sequencing primer AGATCCTCTTCTGAGATG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    PylT-m15_CUA
  • Alt name
    m15-tRNA-Pyl_CUA
  • Promoter proK

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGATTCCTCAAAGCGTAAACAAC
  • 3′ sequencing primer ATGGAACGGGTTGGCATG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.09.636911 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS1T-PylRSC6a-PylTm15CUA was a gift from Alexandria Deliz Liang (Addgene plasmid # 251554 ; http://n2t.net/addgene:251554 ; RRID:Addgene_251554)

  • For your References section:

    Hydrophobic tuning with non-canonical amino acids in a copper metalloenzyme. Deliz Liang A. Unpublished 10.1038/s41557-026-02116-7
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