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pET28(+)-SLAC-6xHis
(Plasmid #251556)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251556 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ScSLAC-H6
  • Alt name
    ScSLAC
  • Alt name
    ScSLAC-His6
  • Alt name
    Small laccase
  • Species
    Streptomyces coelicolor
  • Insert Size (bp)
    969
  • Mutation
    Removed N-terminal signaling peptide; Codon optimized for E. coli
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.09.636911 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28(+)-SLAC-6xHis was a gift from Alexandria Deliz Liang (Addgene plasmid # 251556 ; http://n2t.net/addgene:251556 ; RRID:Addgene_251556)

  • For your References section:

    Hydrophobic tuning with non-canonical amino acids in a copper metalloenzyme. Deliz Liang A. Unpublished 10.1038/s41557-026-02116-7
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