pMJM100
(Plasmid
#252705)
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Purpose(Empty Backbone) E. coli and Lactobacilli shuttle vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTRK829
- Backbone size (bp) 4017
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Modifications to backboneModified cloning vector derived from pTRK829 by inserting a multiple cloning site sequence at StuI and ApaI sites, removing the entire cam expression cassette and other non-essential regions. pTRK829 is a derivative of pGK12, contains an insertion sequence from Laticaseibacillus rhamnosus.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli MC1061
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Growth instructionserythromycin (Em) 5 µg/mL for lactobacilli strains and 200 µg/mL for E. coli.
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Copy numberLow Copy
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMJM100 was a gift from Michael J Miller (Addgene plasmid # 252705 ; http://n2t.net/addgene:252705 ; RRID:Addgene_252705) -
For your References section:
Exploiting the Endogenous Type II-A CRISPR-Cas System for Functional Engineering of Probiotic Lacticaseibacillus rhamnosus GG. Xie Z, Jin YS, Miller MJ. Microb Biotechnol. 2026 Jan;19(1):e70303. doi: 10.1111/1751-7915.70303. 10.1111/1751-7915.70303 PubMed 41556308
Map uploaded by the depositor.
