pN1_RnKIF5C(1-560 d6)-mNG
(Plasmid
#252746)
-
PurposeExpression of a mNG-tagged, truncated (1-560) delta6 KIF5C (kinesin-1). The (1-560) is constitutively active. The delta6 mutant causes excessive damage along the microtubule lattice.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepN1
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKIF5C
-
SpeciesR. norvegicus (rat)
-
Mutationaa 7-560
-
Entrez GeneKif5c
-
Tag
/ Fusion Protein
- mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pN1_RnKIF5C(1-560 d6)-mNG was a gift from Kristen Verhey (Addgene plasmid # 252746 ; http://n2t.net/addgene:252746 ; RRID:Addgene_252746) -
For your References section:
A kinesin-1 variant reveals motor-induced microtubule damage in cells. Budaitis BG, Badieyan S, Yue Y, Blasius TL, Reinemann DN, Lang MJ, Cianfrocco MA, Verhey KJ. Curr Biol. 2022 Jun 6;32(11):2416-2429.e6. doi: 10.1016/j.cub.2022.04.020. Epub 2022 May 2. 10.1016/j.cub.2022.04.020 PubMed 35504282