Lenti-A3Ac-puro
(Plasmid
#253342)
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PurposeExpresses the C-terminal fragment of a rapamycin-inducible split A3A cytosine base editor for in vivo base editing
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneLentiV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameA3Ac
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SpeciesH. sapiens (human)
- Promoter EFS
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Tag
/ Fusion Protein
- 3xFLAG-FKBP12-A3Ac-nCas9-puro
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer AACCGGTGCCTAGAGAAGGT
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-A3Ac-puro was a gift from Junwei Shi (Addgene plasmid # 253342 ; http://n2t.net/addgene:253342 ; RRID:Addgene_253342) -
For your References section:
Inducible, split base editors for in vivo cancer functional genomics. Ren D, Wang S, Yamada K, Liu Y, Hapke R, Alpsoy A, Ho Y, Zhang C, Lan Y, Zhang S, Milazzo JP, Lohia R, Berríos KN, Li Y, Weber EW, Li Q, Vakoc CR, Minn AJ, Kohli RM, Shi J. Nat Biotechnol. 2026 Apr 15. doi: 10.1038/s41587-026-03077-5 10.1038/s41587-026-03077-5