Skip to main content
Addgene
Open Menu

pACT2-DdtS-HsMCU
(Plasmid #253519)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253519 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pACT2
  • Backbone size w/o insert (bp) 7726
  • Total vector size (bp) 8608
  • Modifications to backbone
    Remove the GAL4 activation domain
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HsMCU
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    882
  • GenBank ID
    NM_138357.1
  • Entrez Gene
    MCU (a.k.a. C10orf42, CCDC109A, HsMCU)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (destroyed during cloning)
  • 3′ cloning site Hind III (destroyed during cloning)
  • 5′ sequencing primer AATCAACTCCAAGCTTG ATG AAT AGC TTC GTG ATC AGG AAC GG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACT2-DdtS-HsMCU was a gift from Vamsi Mootha (Addgene plasmid # 253519 ; http://n2t.net/addgene:253519 ; RRID:Addgene_253519)

  • For your References section:

    Reconstitution of the mitochondrial calcium uniporter in yeast. Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber RJ, Myre MA, Blower MD, Mootha VK. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi: 10.1073/pnas.1400514111. Epub 2014 Jun 2. 10.1073/pnas.1400514111 PubMed 24889638
Related items: