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pLYS1- DdMCU_AIIA, Cterm Flag
(Plasmid #253538)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253538 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLys1
  • Backbone size w/o insert (bp) 7390
  • Total vector size (bp) 8215
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DdMCU
  • Species
    Dictyostelium discoideum
  • Insert Size (bp)
    825
  • Mutation
    changed D192A and E195
  • GenBank ID
    XM_632658
  • Entrez Gene
    DDB_G0286429 (a.k.a. DDB_G0286429, DDBDRAFT_0186972, DDB_0186972)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLYS1- DdMCU_AIIA, Cterm Flag was a gift from Vamsi Mootha (Addgene plasmid # 253538 ; http://n2t.net/addgene:253538 ; RRID:Addgene_253538)

  • For your References section:

    Reconstitution of the mitochondrial calcium uniporter in yeast. Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber RJ, Myre MA, Blower MD, Mootha VK. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi: 10.1073/pnas.1400514111. Epub 2014 Jun 2. 10.1073/pnas.1400514111 PubMed 24889638
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