pLV-EF1alpha-FLAG-HA-CAD_WT-HA-IRES-Neo
(Plasmid
#253788)
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PurposepLV lentiviral expression vector encoding FLAG- and HA-tagged human wild-type CAD linked to neomycin selection by an IRES under a human EF1alpha promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLV-EF1alpha-DEST-IRES-Hygro
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Backbone manufacturerThis study
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCAD
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SpeciesH. sapiens (human)
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Entrez GeneCAD (a.k.a. CDG1Z, DEE50, EIEE50, GATD4)
- Promoter EF1alpha
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Tags
/ Fusion Proteins
- FLAG-HA (N terminal on insert)
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-EF1alpha-FLAG-HA-CAD_WT-HA-IRES-Neo was a gift from Samuel McBrayer (Addgene plasmid # 253788 ; http://n2t.net/addgene:253788 ; RRID:Addgene_253788)
