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-2.5CAT
(Plasmid #25424)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25424 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 25424-D50 50 μg of DNA in Tris buffer $495
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DNA 25424-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCATdeltaEH
  • Backbone manufacturer
    Mellon Lab
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression ; Reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myo D Promoter
  • Alt name
    MyoD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Entrez Gene
    MYOD1 (a.k.a. CMYO17, CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
  • Tag / Fusion Protein
    • CAT (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer CAT-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The -2.5 promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 6.8kb.

All tk sequences were removed from the CAT vector in the construction of -2.5kb.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -2.5CAT was a gift from Charles P. Emerson Jr. (Addgene plasmid # 25424 ; http://n2t.net/addgene:25424 ; RRID:Addgene_25424)

  • For your References section:

    Regulatory elements that control the lineage-specific expression of myoD. Goldhamer DJ, Faerman A, Shani M, Emerson CP. Science. 1992 Apr 24. 256(5056):538-42. 10.1126/science.1315077 PubMed 1315077
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