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(Plasmid #25425)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 25425 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Mellon Lab
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression ; Reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Myo D Promoter + F3
  • Alt name
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
  • Tag / Fusion Protein
    • CAT (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer CAT-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fragment 3 was cloned into the XbaI site of -2.5CAT by digesting chMD-13 with NotI and EcoRI, and blunt-end-ligating the fragment into the unique XbaI site.

The -2.5 promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 6.8kb.

All tk sequences were removed from the CAT vector in the construction of -2.5kb.

Between the enhancer and promoter is a portion of pBluescript polylinker that includes BamHI, SpeI and SmaI sites, none of which are unique.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    F3/-2.5CAT was a gift from Charles P. Emerson Jr. (Addgene plasmid # 25425 ; ; RRID:Addgene_25425)
  • For your References section:

    Regulatory elements that control the lineage-specific expression of myoD. Goldhamer DJ, Faerman A, Shani M, Emerson CP. Science. 1992 Apr 24. 256(5056):538-42. 10.1126/science.1315077 PubMed 1315077