Fta RNA polymerase
(Plasmid #254269)

• Purpose: Encodes E. coli codon-optimized rpoA, rpoB, rpoC, and rpoZ from Flagellimonas taeanensis driven by an inducible T7 promoter
• Depositing Lab: Samuel H. Sternberg
• Publication: Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-026-10166-7.

Backbone

• Vector backbone: pACYCDuet
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: rpoA
• Species: Flagellimonas taeanensis
• Insert Size (bp): 993
• GenBank ID: RIV50261.1
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATGGCTCTATTAAATTTTCAAAAACCCGATAA

Gene/Insert 2

• Gene/Insert name: rpoB
• Species: Flagellimonas taeanensis
• Insert Size (bp): 3810
• GenBank ID: RIV49782.1
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TCCCAAGGAGACGTACCATG

Gene/Insert 3

• Gene/Insert name: rpoC
• Species: Flagellimonas taeanensis
• Insert Size (bp): 4299
• GenBank ID: RIV49783.1
• Promoter: T7

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAGGAGATATACATATGGCAAGGATAAA

Gene/Insert 4

• Gene/Insert name: rpoZ
• Species: Flagellimonas taeanensis
• Insert Size (bp): 327
• GenBank ID: RIV49301.1
• Promoter: T7

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAGACGTACCATGCAAGATTTAAAGAATACAA

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