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dCas12f–σE (sigmaE) system, entry vector
(Plasmid #254272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254272 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDF
  • Backbone size w/o insert (bp) 3191
  • Total vector size (bp) 6024
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    dCas12f
  • Species
    Flagellimonas taeanensis
  • Insert Size (bp)
    1098
  • GenBank ID
    WP_119619048.1
  • Promoter J23105

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sigma E
  • Species
    Flagellimonas taeanensis
  • Insert Size (bp)
    786
  • GenBank ID
    RIV51358.1
  • Promoter J23105

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas12f–σE (sigmaE) system, entry vector was a gift from Samuel H. Sternberg (Addgene plasmid # 254272 ; http://n2t.net/addgene:254272 ; RRID:Addgene_254272)

  • For your References section:

    Exapted CRISPR-Cas12f homologues drive RNA-guided transcription. Hoffmann FT, Wiegand T, Palmieri AI, Glass-Klaiber J, Xiao R, Tang S, Le HC, Meers C, Lampe GD, Chang L, Sternberg SH. Nature. 2026 Mar 4. doi: 10.1038/s41586-026-10166-7. 10.1038/s41586-026-10166-7 PubMed 41781627
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