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pMIRNA1-METTL1-msg6-EIR107-109AAA
(Plasmid #254423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254423 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMIRNA1
  • Vector type
    Lentiviral
  • Selectable markers
    CopGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    METTL1
  • Alt name
    TRMT8
  • Alt name
    C12orf1
  • Species
    H. sapiens (human)
  • Mutation
    EIR107-109AAA
  • Entrez Gene
    METTL1 (a.k.a. C12orf1, TRM8, TRMT8, YDL201w)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3_FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIRNA1-METTL1-msg6-EIR107-109AAA was a gift from Rui Su (Addgene plasmid # 254423 ; http://n2t.net/addgene:254423 ; RRID:Addgene_254423)

  • For your References section:

    Targeting the METTL1/m7G axis as a therapeutic strategy in myeloid leukemia. Ren L, Zhang H, Bobileva O, Nai F, Bi H, Baker G, Dong L, Guarin D, Chan A, Hu W, Li W, Leite I, Wang X, Zhang X, Xue M, Wang H, Qin H, Wu X, Ghoda L, Xu L, Zhang B, Li L, Wunderlich M, Mulloy J, Jones C, O'Leary S, Rosen S, Chen C, Heisterkamp N, Perry JJ, Nam Y, Chen J, Li X, Caflisch A, Su R. Blood 2025; 146 (Supplement 1): 2539. doi: 10.1182/blood-2025-2539 10.1182/blood-2025-2539
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