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pLKO.1-shtRNA-Phe(GAA)-2
(Plasmid #254427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254427 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Bob Weinberg, Addgene Plasmid #8453
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tRNA-Phe (anticodon GAA) 1-4
  • Alt name
    TRNAF2
  • Alt name
    TRF-GAA1-2
  • gRNA/shRNA sequence
    GTTAGACTGAAGATCTAAAGG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    21
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CCGGGTTAGACTGAAGATCTAAAGGCTCGAGCCTTTAGATCTTCAGTCTAACTTTTTG
  • 3′ sequencing primer AATTCAAAAAGTTAGACTGAAGATCTAAAGGCTCGAGCCTTTAGATCTTCAGTCTAAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1-shtRNA-Phe(GAA)-2 was a gift from Rui Su (Addgene plasmid # 254427 ; http://n2t.net/addgene:254427 ; RRID:Addgene_254427)

  • For your References section:

    Targeting the METTL1/m7G axis as a therapeutic strategy in myeloid leukemia. Ren L, Zhang H, Bobileva O, Nai F, Bi H, Baker G, Dong L, Guarin D, Chan A, Hu W, Li W, Leite I, Wang X, Zhang X, Xue M, Wang H, Qin H, Wu X, Ghoda L, Xu L, Zhang B, Li L, Wunderlich M, Mulloy J, Jones C, O'Leary S, Rosen S, Chen C, Heisterkamp N, Perry JJ, Nam Y, Chen J, Li X, Caflisch A, Su R. Blood 2025; 146 (Supplement 1): 2539. doi: 10.1182/blood-2025-2539 10.1182/blood-2025-2539
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