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pT2K-CAGGSv3-human SMART
(Plasmid #256109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 256109 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pT2K-CAGGSv3-EGFP
  • Backbone manufacturer
    K. Kawakami
  • Backbone size w/o insert (bp) 8960
  • Total vector size (bp) 8126
  • Vector type
    Mammalian Expression ; transposon-based vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ypet-MLKL-ECFP
  • Alt name
    Mixed lineage kinase domain-like
  • Species
    H. sapiens (human); Aequorea victoria
  • GenBank ID
    XM_047433704.1
  • Entrez Gene
    MLKL (a.k.a. hMLKL)
  • Promoter CAG
  • Tags / Fusion Proteins
    • Ypet (N terminal on insert)
    • ECFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer cgccggcaggaaggaaat
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2K-CAGGSv3-human SMART was a gift from Hiroyasu Nakano (Addgene plasmid # 256109 ; http://n2t.net/addgene:256109 ; RRID:Addgene_256109)

  • For your References section:

    A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs. Murai S, Yamaguchi Y, Shirasaki Y, Yamagishi M, Shindo R, Hildebrand JM, Miura R, Nakabayashi O, Totsuka M, Tomida T, Adachi-Akahane S, Uemura S, Silke J, Yagita H, Miura M, Nakano H. Nat Commun. 2018 Oct 26;9(1):4457. doi: 10.1038/s41467-018-06985-6. 10.1038/s41467-018-06985-6 PubMed 30367066
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