Purpose(Empty Backbone) Entry vector for cloning miR30-based shRNA driven by human U6 promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25747||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerATCC 10326362
- Backbone size (bp) 4608
Vector typeEntry vector
Growth in Bacteria
Bacterial Resistance(s)Gentamicin, 10 μg/mL
Growth instructionsDB3.1 (ccdB survival)
- Cloning method Restriction Enzyme
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attL2 (not destroyed)
- 5′ sequencing primer hU6-F (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byU6 promoter and miR-30- Greg Hannon, CSHL
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
shRNA expression; miR30-based topology; Human U6 promoter; 27nt U6 leader sequence expressed in transcript; Entry vector backbone; +ccdB in parent;
Addgene sequence shows that there is a T missing in U6 promoter sequence. Depositor is aware of the mismatch and it should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEN_hUmiRc2 was a gift from Iain Fraser (Addgene plasmid # 25747 ; http://n2t.net/addgene:25747 ; RRID:Addgene_25747)
For your References section:A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906