pSO2002
(Plasmid #25842)

• Depositing Lab: Aziz Sancar
• Publication: Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-32.

Backbone

• Vector backbone: pcDNA4
• Backbone size w/o insert (bp): 5274
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Cryptochrome 2
• Alt name: hCry2
• Species: H. sapiens (human)
• Insert Size (bp): 1783
• Entrez Gene: CRY2 (a.k.a. HCRY2, PHLL2)
• Tags / Fusion Proteins:
  • flag (C terminal on insert)
  • myc (C terminal on backbone)
  • his (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (unknown if destroyed)
• 3′ cloning site: BstBI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry