sVdAvi-GAB1
(Plasmid
#259390)
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PurposeSubstrate co-expression for phosphorylation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonesVd (pGEX mod)
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Backbone manufacturerSigma Aldrich
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAB1 Y627/Y659
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SpeciesH. sapiens (human)
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Tags
/ Fusion Proteins
- msmt3-p40 (N terminal on backbone)
- AviTag-Myc-6xHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SacII (not destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMatt Lazzara, pCDNA3.1-HA-GAB1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.22.629992 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sVdAvi-GAB1 was a gift from Kristen Naegle (Addgene plasmid # 259390)