pETSF-PD1_Y248
(Plasmid
#259394)
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PurposeSubstrate co-expression for phosphorylation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET
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Backbone manufacturerTwist
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePD1 Y248
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SpeciesH. sapiens (human)
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Tags
/ Fusion Proteins
- SUMO2m-p40F-Linker2-p40F (N terminal on insert)
- Fh8-StrepTagII-Myc-6xHis (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.22.629992 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETSF-PD1_Y248 was a gift from Kristen Naegle (Addgene plasmid # 259394)