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sVf-PTPN11_NSH2
(Plasmid #259397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259397 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    sVd (pGEX mod)
  • Backbone manufacturer
    Sigma Aldrich
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTPN11 N_SH2
  • Species
    H. sapiens (human)
  • Tags / Fusion Proteins
    • 10xHis-msmt3 (N terminal on insert)
    • Myc-KExLinker-p40W (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacII (not destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Matt Lazzara, pBABE-PTPN11

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.22.629992 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sVf-PTPN11_NSH2 was a gift from Kristen Naegle (Addgene plasmid # 259397)
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