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pLenti6.3-EML4-ALK
(Plasmid #259519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259519 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLenti6.3/TO/V5-DEST
  • Backbone manufacturer
    Invitrogen (Thermo Fisher Scientific)
  • Backbone size w/o insert (bp) 7521
  • Total vector size (bp) 10724
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EML4-ALK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3203
  • Entrez Gene
    EML4 (a.k.a. C2orf2, ELP120, EMAP-4, EMAPL4, ROPP120)
  • Entrez Gene
    ALK (a.k.a. ALK1, CD246, NBLST3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site Mlu I (destroyed during cloning)
  • 5′ sequencing primer CMV-Forward
  • 3′ sequencing primer Internal
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3-EML4-ALK was a gift from Curtis Harris & Izumi Horikawa (Addgene plasmid # 259519 ; http://n2t.net/addgene:259519 ; RRID:Addgene_259519)

  • For your References section:

    EML4-ALK induces cellular senescence in mortal normal human cells and promotes anchorage-independent growth in hTERT-transduced normal human cells. Miyanaga A, Matsumoto M, Beck JA, Horikawa I, Oike T, Okayama H, Tanaka H, Burkett SS, Robles AI, Khan M, Lissa D, Seike M, Gemma A, Mano H, Harris CC. BMC Cancer. 2021 Mar 24;21(1):310. doi: 10.1186/s12885-021-07905-6. 10.1186/s12885-021-07905-6 PubMed 33761896
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